The role of internal transcribed spacer 2 secondary structures in classifying mycoparasitic Ampelomyces
نویسندگان
چکیده
منابع مشابه
A common core of secondary structure of the internal transcribed spacer 2 (ITS2) throughout the Eukaryota.
The ongoing characterization of novel species creates the need for a molecular marker which can be used for species- and, simultaneously, for mega-systematics. Recently, the use of the internal transcribed spacer 2 (ITS2) sequence was suggested, as it shows a high divergence in sequence with an assumed conservation in structure. This hypothesis was mainly based on small-scale analyses, comparin...
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Ticks are important acarina that infest animals. They are obligatory blood sucker arthropods which economically impact cattle industry by reducing weight gain and production. Moreover, they are important vectors of viral, bacterial, rickettsial and parasitic pathogens infecting humans and animals. In view of the importance of Hyalomma anatolicum anatolicum in pathogen transmission, including Th...
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Lettuce is belong to the Cichorium family with leaf consumption, Annual cold weather plants and rich in minerals, vitamins and nutrients. The aim of the present study was to evaluate the genetic diversity of 15 varieties and lettuce populations using the ITS area. Sequence quality was evaluated using Chromas 2.1.1 software, aligned by MegAlign 5 software and then similarity matrix and dendrogra...
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Sequences of the nuclear internal transcribed spacer (ITS) regions ITS1 and ITS2 have been used widely in molecular phylogenetic studies because of their relatively high variability and facility of amplification. For phylogenetic applications, most researchers use sequence alignments that are based on nucleotide similarity. However, confidence in the alignment often deteriorates at taxonomic le...
متن کاملAre similarity- or phylogeny-based methods more appropriate for classifying internal transcribed spacer (ITS) metagenomic amplicons?
• The internal transcribed spacer (ITS) of the nuclear ribosomal DNA region is a widely used species marker for plants and fungi. Recent metagenomic studies using next-generation sequencing, however, generate only partial ITS sequences. Here we compare the performance of partial and full-length ITS sequences with several classification methods. • We compiled a full-length ITS data set and creat...
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ژورنال
عنوان ژورنال: PLOS ONE
سال: 2021
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0253772